1 Noll 2020 · The successful application of monoclonal antibodies (mAb) in oncology and autoimmune diseases paved the way for the development of therapeutic antibod…

To evaluate the buffer preparation reproducibility for salt- and pH-based IEX eluents, phosphate salt buffers, MES salt buffers, and CX-1 pH gradient buffers were prepared by two different analysts on …

22 Samh 2023 · Recently, the use of mass spectrometry-compatible salt-mediated pH gradients has gained increased attention to elute the proteins from the charged stationary phase material. However, …

It reviews the range of counterions used in pharmaceutical salts, including their chemical characteristics and the rationale for their selection. The methods for preparing pharmaceutical salts are examined in …

28 DFómh 2017 · A roadmap for the selection of a pharmaceutical salt form for a development candidate is presented. The free base of the candidate did not have sufficient chemical stability for …

21 Beal 2025 · Purification was carried out on a strong anion exchanger using a phosphate buffer and a linear salt gradient. We focus on the impact of solution pH (8.0 to 12.0) and elution salt type (sodium …

In this study, the use of Waters Atlantis Premier BEH Z-HILIC Column in combination with ELSD was shown to be successful at retaining and separating various counterions in salts, as well as separating …

8 Feabh 2025 · Ion exchange chromatography (IEX) is an important analytical technique for the characterization of biotechnology-derived products, such as monoclonal …

As with media selection, the choice of salt for a HIC separation can be a matter of trial and error since each salt differs in its ability to promote hydrophobic interactions.

Evaluation and application of salt- and pH-based ion-exchange chromatography gradients for analysis of therapeutic monoclonal antibodies This study demonstrates the use of a pH buffer system for charge …