Learn the comprehensive method of whole blood staining and preparation for flow cytometry analysis with cell surface markers.

This protocol provides an overview of how to perform flow cytometric staining of whole blood samples, including steps on red blood cell lysis, surface marker staining, and fixation.

Collect normal or donor blood in the presence of anticoagulant. Depending on how many cells need to be collected, each staining tube will need 100–300 μL of blood.

2022年12月16日 · To maximize MC data quality, we present a protocol for whole blood analysis together with an R package, Cyto Quality Pipeline (CytoQP), which minimizes the experimental …

Add predetermined optimum concentrations of desired fluorochrome conjugated, biotinylated, or purified primary antibodies to 100μl of anti-coagulated whole blood.

Refer to Cytek® 25-Color Immunoprofiling Assay Acquisition Protocol for a step-by-step guide for sample acquisition and analysis in SpectroFlo®. Please note that this kit is designed for research use …

The Intracellular Staining With True-Phos Perm Buffer in Whole Blood Protocol describes the process of intracelluar staining of cells in whole blood using Biolegend's proprietary buffers and antibodies.

This protocol describes how to perform whole blood staining for flow cytometry analysis of cell surface markers.