Restriction Digest of Plasmid DNA
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Restriction Digest of Plasmid DNA
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Title: Restriction Digest/Ligation WorksheetPage 2 of 2In thi... | Filo
Title: Restriction Digest/Ligation WorksheetPage 2 of 2In thi... | Filo
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SOLVED: You have performed a restriction digest on a linear 10.5 Kbp piece of DNA. The sizes of the DNA fragments resulting from the different restriction digests are shown below. Using this information, construct a linear map of this DNA illustrating the location(s) of the different restriction enzyme sites and the distances between each site. (20 pts)Enzyme Size of Fragments Produced (Kbp)BamHI 6.5, 2.5, 1.5EcoRI 5.5, 3.0, 2.0SalI 10.0, 0.5HindIII 8.5, 2.0BamHI/EcoRI 4.0, 2.5, 2.0, 1.5, 0.5Bam
SOLVED: You have performed a restriction digest on a linear 10.5 Kb…
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